Zeiss Axio Observer 3 Fluorescence Microscope
Used Zeiss Axio Observer 3 Inverted Phase Contrast Fluorescence Microscope
- Used Condition
- Genuine Product
- Manufacturer Warranty
- Technical Support
- Worldwide Shipping
Selling Pre-owned Zeiss Axio Observer 3 Fluorescence Microscope with Excelitas X-Cite LED Fluorescence. Objectives upgrade PI 10x/23, LD Plan-Neofluar 40x/0,6 Ph2 Korr, LD Plan-Neofluar 20x/0,4 Ph2 Korr, EC Plan-Neofluar10x/0,3 Ph1, N Achroplan 5x/0,13 Ph0, N Achroplan 2,5x/0,07.
The item has been tested and is in good working condition. Comes with a 30-day money back guarantee. We take responsibility until the packages reach destination with protected, robust wooden crating system and shipped worldwide.
Package includes:
- Zeiss Axio Observer 3 Inverted Microscope Stand (Model 431004-9901-000)
- Trinocular Viewing Head with C-Mount Camera Port
- Manual Encoded 6-Position Nosepiece
- Manual Encoded Fluorescence Filter Cube Turret
- Manual Condenser with Phase Contrast Annuli
- Fluorescence Illuminator: 100w Mercury with Power Supply
- (3) Fluorescence Filter Cubes
- Transmitted Light Module (Halogen)
- Power Supply and Cabling
- 30-day money abck guarantee
The Zeiss Axio Observer 3 (Model 431004-9901-000) is a manual research-grade inverted microscope equipped for phase contrast and multi-channel fluorescence imaging. Built with Zeiss’s renowned optics and encoded components for repeatable documentation, this model is ideal for fixed-cell fluorescence, morphology, and slide-based workflows. Designed for versatility and precision, the Observer 3 includes a trinocular head and modular transmitted and fluorescence light paths. Stage not included by default — ready to be customized for your application.
Key Features
- Manual microscope with encoded turret and nosepiece
- Fluorescence and phase contrast imaging modes
- Ideal for fixed-cell imaging, teaching, and documentation
- Modular light paths for transmitted and fluorescence workflows
- Designed for slide, plate, and dish-based sample formats
Applications
- Fixed Cell Fluorescence Imaging
- Phase Contrast for Cell Morphology
- Multi-Channel Fluorescence Documentation
- Immunofluorescence Assays
- Research, Education, and Imaging Labs
Zeiss Axio Observer 3 Specifications
| Microscope stands inverted | Microscope stand Axio Observer 3 - 6-position nosepiece, H DIC M27 cod. - manual focus drive - 1-position tube lens mount with 1x tube lens - path deflection to the tube for visual observation - slider mount for PlasDIC and analyzer module - keys for switching TL/RL illumination - light manager - interfaces: 1x USB and 1x CAN - integrated 12V DC 100W power supply, stabilized, 200V-240VAC / 50-60Hz / max. 300VA - USB cable and country-specific line cable |
| Sideport 60N L, 2 switching positions for basic stands Axio Observer 3 / 5 / A1 / D1 (100% vis : 0% L / 20% vis : 80% L) | |
| Aqua Stop II for Axio Observer, Axiovert 5 and Axiovert 200 | |
| Transmitted-light illumination | Carrier for transmitted-light illumination tiltable, for the mounting of condensers. Equiped with luminous-field diaphragm, 3-position filter changer and attachment possibility for micromanipulators. |
| Lamps and Lamp housings | Illuminator microLED 3 for mounting on the lamp interface for transmitted-light or reflected-light illumination - colour temperature 5700 K - colour rendering index >90 |
| Light Source Viluma 7 RGB-UV 4-channel fluorescence light source with integrated control unit for continuous brightness adjustment, quickly switchable and stable on the long term. With integrated monitor diode for repetitious brightnessadjustment. Equipped with 4 solid state LED lamps. Red (631 nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes Green (555 nm) for excitation of Cy3, TRITC, DsRed and similar dyes Blue (469 nm) for excitation of eGFP, Fluo4, FITC and similar dyes UV(385 nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes A country-specific power cable and a desktop power supply are included in delivery. When used at an Axio Observer with Incubator XLmulti, the Extension tube for illuminator mount, 17 mm is required. | |
| Control Panel for Colibri 5/7 and Viluma 5/7 with LCD to display the operating state. For LED selection and brightness setting. | |
| Stages and accessories | Specimen stage 250x230 mm with hardcoat anodized surface |
| Universal mounting frame M for: - Petri dishes dia. 24...68 mm - slides (max. length 120 mm) | |
| Object guide 130x85 mm right can be attached to both sides, accepts various mounting frames M | |
| Tubes and accessories | Binocular tube 45°/23 with manual shutter |
| Eyepieces | Eyepiece PL 10x/23 Br. foc. With mount for reticle diam. 26 mm. |
| Auxiliary microscope, d=30 to adjust the phase rings and darkfield stop in the condenser | |
| Eyepiece accessories | Eyepiece eyecup |
| Transmitted-light objectives | Objective LD A-Plan 10x/0.25 Ph1 M27 (FWD=8.5mm at CG=1mm polystyrene) |
| Objective LD Plan-Neofluar 20x/0.4 Corr Ph2 M27 (CG=0-1.5mm) (FWD=8.4mm at CG=0mm and FWD=7.4mm at CG=1.5mm) | |
| Objective LD Plan-Neofluar 40x/0.6 Corr Ph2 M27 (CG=0-1.5mm) (FWD=3.3mm at CG=0mm and FWD=2.5mm at CG=1.5mm) | |
| Objective LD A-Plan 5x/0.15 M27 (FWD=11.7mm at CG=1mm polystyrene) | |
| Condensers | LD condenser 0.55 H, Ph1, Ph2, Ph3, DIC, DIC; 6 positions with aperture diaphragm, WD=26mm For objective magnifications 4.0x-100x |
| Stand accessories | 6-position reflector turret man. for P&C modules reflector modules exchangable without removal of reflector turret |
| Protection filter for reflector turret | |
| Reflected-light illumination | Reflected-light illuminator FL - slider mounts for manual sliders - slider mount for 3-position filter slider, dia. 25 mm - beam path apochromatic corrected - transmission from 375 to 825 nm >80% |
| Antiglare screen for Axio Observer/Axiovert | |
| Iris stop slider for incident-light equipment centerable, can be used as luminous-field diaphragm and/or aperture diaphragm slider (Köhler-type illumination in incident-light beam path | |
| Reflector module FL EC P&C Reflection avoided through tilted mount for emission filters, max. filter thickness: 5 mm | |
| Filters | Conversion filter 5700-3200 K, d=32 mm for adapting the LED light to the color impression of halogen light |
| Filter set 90 HE LED (E) for use with ZEISS LED fluorescence light sources. Suitable for fluorescent dyes like DAPI, FITC, TRITC and Cy5 with excitation wavelengths 385, 475, 555 and 630 nm. Contains beam splitter QBS 405 + 493 + 575 + 653 and emission filter QBP 425/30 + 514/30 + 592/25 + 709/100. | |
| Dimensions (W x L x H) | :295 x 805 x 707 mm |
| Weight | 27 Kg |